Skip to content

For research and pharmaceutical organizations only. No consumer sales.

Solenyx Logo
RNA regulation & analogs

miRNA mimics and inhibitors, PMO and aptamers

Oligonucleotides beyond classic ASO/siRNA: double-stranded miRNA mimics, single-stranded anti-miRs, phosphorodiamidate morpholino oligomers (PMO), and DNA or 2'-modified aptamers for target binding. Each class uses a distinct backbone and analytics approach.

All oligonucleotides

miR-21–23

mer mimic bands (typical)

Morpholino

PMO backbone

25–60

nt aptamer ranges

SELEX

aptamer discovery path

Steric

PMO / anti-miR mechanism

miRNA, PMO and aptamer roles

miRNA mimics restore or augment miRNA function with a passenger-like and guide-like strand; inhibitors (anti-miRs) block mature miRNA with high-affinity complementary sequences, often with heavy 2'- modification.

PMOs use uncharged phosphorodiamidate morpholino linkages for steric interference with splicing or translation without recruiting RNase H. Aptamers fold into structured binders selected against proteins or small molecules, then manufactured as synthetic oligos with chemical stability motifs.

Selecting the modality

Biological taskTypical formatKey chemistry
Restore miRNA levelsDuplex mimic2'-OMe / phosphorothioate pattern; passenger mismatch design
Silence a mature miRNAAnti-miR (ss)High-affinity tail; often PS backbone
Exon skip in Duchenne-like modelsPMOMorpholino; no PS; charge-neutral
Bind protein / epitopeAptamer2'-F, 2'-NH2, inverted dT caps; SELEX-derived sequence

Figures are indicative industry-typical values. Lot- and project-specific data appear on the quotation and certificate of analysis.

Indicative specifications by format

miRNA mimic

Duplex with defined passenger

Similar QC to siRNA

Anti-miR

Single strand

Identity by MS; nuclease-stable pattern

PMO

Morpholino oligomer

Mass confirms length; CE or HPLC purity

Aptamer

Structured ssDNA/RNA analog

Folding buffer noted on COA

Purity

≥85–95%

Higher for in vivo / GMP

Identity

MS primary

Sequence as provided

Figures are indicative industry-typical values. Lot- and project-specific data appear on the quotation and certificate of analysis.

Format catalog

miRNA tools

  • Mature miRNA mimics (dual strand)
  • Seed-targeting inhibitors
  • miRNA sponges (longer constructs, project-specific)

PMO

  • Splice-switching morpholinos
  • Translation-blocking PMO
  • End-modified PMO (peptide conjugates via conjugates page)

Aptamers

  • 2'-fluoro pyrimidine aptamers
  • 2'-amino or 2'-OMe mixed motifs
  • PEG or biotin terminal tags (labels page)

What you receive

  • Material per format: lyophilized or liquid
  • COA with identity, purity and noted salt / buffer form
  • Duplex-specific data for mimics; single-strand MS for anti-miRs and aptamers
  • PMO: chromatographic purity profile typical
  • Handling notes for folding (aptamers) or PMO solubility
  • Quote referencing length, backbone and scale

Frequently asked questions

Mimics are designed to replace or augment endogenous miRNA function, including passenger strand design rules. Manufacturing and QC resemble siRNA but sequence and modification maps follow miRNA biology.

Related

Antisense (ASO)

Single-stranded phosphorothioate-rich ASOs for RNase H–mediated knockdown (gapmers) or sequence-specific binding without degradation (steric blockers). Chemistry, length and modification pattern are chosen to the target transcript, tissue and intended clinical dose.

View details

Peptide & Lipid Conjugates

Oligos linked to cell-penetrating peptides (CPP), peptide-PMO (PPMO) constructs, cholesterol and other lipid anchors, and miscellaneous delivery ligands. Conjugation chemistry preserves oligo integrity while enabling uptake in muscle, CNS and other tissues beyond GalNAc-liver programs.

View details

Backbone & Sugar Modifications

Phosphorothioate (PS) backbones and 2'- sugar patterns (2'-OMe, 2'-F, 2'-MOE, LNA) tune nuclease resistance, affinity, innate immunity and tolerability. Modification maps are applied per position during solid-phase synthesis.

View details

Labels & Dyes

Oligonucleotides conjugated at the 5', 3' or internal positions with fluorophores, dark quenchers, biotin or other haptens for qPCR probes, FRET assays, imaging and pull-down. Compatibility with the underlying PS/2' map is validated during synthesis.

View details
Next step

Ready to specify this for your program?

Share sequence, scale and documentation needs. We reply with indicative specs and a project-specific quotation.